Identification of
Up-regulated Genes in Rat Regenerating Liver Tissue by Suppression Subtractive
Hybridization
LIU Zhan-Wu, ZHAO Mu-Jun*, LI Zai-Ping
( Institute of Biochemistry and Cell Biology, Shanghai Institutes for
Biological Sciences, �–hinese Academy of Sciences, Shanghai 200031,
China )
Abstract Suppression
subtractive hybridization (SSH) technique was used to screen up-regulated genes
in regenerating liver. The cDNA from rat regenerating liver tissue was used as
the tester, and that from normal liver was used as the driver. After cloning, a
subtraction cDNA library of 900 clones was obtained. These 900 clones were
further analyzed by differential gene expression screening, and 50 clones with
obvious overexpression in regenerating liver were identified. Sequencing
analysis and homology searching showed that these clones represent 37 genes, 13
of them are homologous to genes known to be involved in liver regeneration； 15 are known genes which are
first found to be up-regulated in regenerating liver； and 9 are novel genes (ESTs)
which have been deposited to GenBank. RNA dot blotting analysis was used to
further study the gene expression patterns. The results not only confirmed the
up-regulation of these genes in regenerating liver tissue, but also showed that
these genes had different expression patterns during the process of liver
regeneration. The results implied that these genes might play important roles
in liver regeneration.
Key words gene screening； liver regeneration； suppression subtractive
hybridization
*Corresponding Author： Tel, 86-21-64374430； Fax, 86-21-64338357； e-mail, [email protected]