Expression of Human
Single Chain Interleukin 12 Gene in Drosophila Cells
JIN Li*, QI Xu1, LAI Bao-Chang1,
SI Lü-Sheng1, WANG Yi-Li1
( Central Laboratory of Shanghai No.1 Hospital, Shanghai 200080,
China；1Medical College, Xi’an Jiao Tong
University, Xi’an 710061,
China )
Abstract The hscIL-12
gene(no signal sequence), amplified by PCR, was subcloned into an expressing
vector pMT/Bip/V5. The recombinant plasmid was transferedinto Drosophila
cell line S2. The secreted hscIL-12 fusion protein was identified by Western
blot, and the bioactivity of hscIL-12 was investigated by lymphocyte
proliferation assay. The results showed hscIL-12 expressed in S2 cells could
stimulate lymphocyte proliferation in dose-dependent manner. hscIL-12 has
bioactivity identical to natural interleukin 12. These findings suggest that
hscIL-12 is promising in theoretical reseach and in clinical use.
Key words interleukin 12； bioactivity； gene expression
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