Effects of Cyclosporine
A and Highly Expressed Bcl-2 on Apoptosis of HL-60 Cells Induced by EGTA
MA Jing, FU Nai-Yang1, XU An-Long*
( Department of Biochemistry and Center for Biopharmaceutical Research,
College of Life Science, Zhongshan University, Guangzhou 510275, China；1Guangdong Branch of China
Institute of Imported and Exported Drug Control, Guangzhou 510180, China )
Abstract Effects of
mitochondrial permeability transition pore specific inhibitor cyclosporine A
(CsA) and highly expressed Bcl-2 on the apoptosis of HL-60 cells induced by
EGTA were studied. Detection of apoptotic peak by flow cytometry, fluorescent
microscope observation of chromatin condensation with double staining of PI and
Hoechst33342 and DNA ladder analysis all demonstrated that CsA obviously
enhanced the apoptosis of HL-60 cells induced by EGTA, while highly expressed
Bcl-2 completely blocked it. It is revealed by mitochondrial membrane potential
(Δψm) fluorescent probes rhodamine 123 and CMXRos that the Δψm
decreased in the apoptosis of HL-60 cells induced by EGTA. CsA enhanced the
decrease of Δψm, but highly expressed Bcl-2 increased Δψm
of HL-60 cells about 2-fold and completely blocked the decrease of Δψm.
Key words apoptosis； mitochondrial permeability
transition pore； cyclosporine A； Bcl-2； HL-60
*Corresponding author： Tel, 86-20-84113655； e-mail, [email protected]