Hypochlorite and Cu2＋ Induced Oxidation of
High Density Lipoprotein
FU Qiang, LIU
Bing-Wen*
( Apolipoproteins Research Unit, Institute of Biochemistry and Molecular
Biology, West China University of Medical Sciences, Chengdu 610041, China
)
Abstract In order to
further study on the properties of oxidatively modified high density
lipoproteins(Ox-HDL), the effects of hypochlorite and Cu2＋ on HDL oxidation in vitro
were investigated. The results showed that, as to LDL, HDL was also oxidized by
hypochlorite and Cu2＋, causing the formation of
thiobarbituric acid substances (TBARS) and increase of negative charges on HDL
particle surface. It was also found that during the oxidation of hypochlorite,
both apoAI and apoAII were degraded into small fragments, the
phosphatidylcholine(PC) hydrolyzed into lysophosphatidylcholine (LPC) and the
protein components of HDL formed carbonyls.
Key words high density lipoprotein； oxidative modification； hypochlorite； Cu2＋
*Corresponding author： Tel, 86-28-5501289； Fax, 86-28-5583252； e-mail, [email protected]