The Spot Fitting and
Expression Quantification for the Nylon Filter cDNA Microarray
ZHANG Wen,
DING Da-Fu*
( Institute of Biochemistry and Cell Biology, Shanghai Institutes for
Biological Sciences, the Chinese Academy of Sciences, Shanghai 200031,
China )
Abstract
High-density cDNA microarray is important in monitoring large scalegenomic
expression profile. With radiolabeled cDNA sample, nylon filter cDNA
microarrays have high sensitivity and wide linear range of hybridization
signals, so it can be employed to detect many important low abundance cDNAs.
However, the nylon filter tends to be randomly contaminated, and the array
spots are prone to dispersion and saturation, resulting in inaccurate
expression value. Based on aphysical model, we address a novel treatment with
accurate positioning, complement of saturation and correction of interference.
Thus the accurate quantificati on of expression can be extracted with
remarkably improved reproducibility.
Key words spot fitting； image analysis； accurate position； complement of saturation； correction of interference
*Corresponding author： Tel, 86-21-64374430； Fax, 86-21-64338357； e-mail, [email protected]