Cloning and Expression
Analysis of a Laryngeal Carcinoma Related Gene, LCRG1
LI You-Jun,
XIE Hai-Long, CHEN Zhu-Chu*, HE Chun-Mei
( Cancer Research Institution of XiangYa Medical School of Central Southern
Universituy, Changsha 410078, China )
Abstract By
screening a human adult cDNA library using a cDNA fragment (AF10056) as a
probe, which is significantly down-regulated in laryngeal carcinoma and
represents a novel gene, a cDNA, LCRG1(laryngeal carcinoma related
gene 1) was identified, which was significantly down-regulated in 12 of 30(40%)
primary laryngeal carcinomas and in 6 of 11(54.5%) various cancer cell lines.
This gene, localized on chromosome band 17q12–21.1 by alignment of it with STS
markers, was composed of six exons and spaned about 60 kb of genomic DNA with a
3.4 kb mature transcript. The putative protein encoded by this gene was 288
amino acid with no significant homology with any known proteins in databases. LCRG1
was expressed in many tissues, as shown by MTN blot analysis. These data
suggest that LCRG1 is related to the laryngeal carcinoma.
Key words laryngeal carcinoma；LCRG1；cloning；expression analysis
*Corresponding author： Tel, 86-731-4805447；Fax, 86-731-4485482； e-mail, [email protected]