Diversity of PLA2 Genes from
Sea Snake Lapemis hardwickii Gray Venom
YANG Wen-Li,
WEI Jian-Wen, ZHONG Xiao-Fen, ZHAO Gui-Jun, PENG Li-Sheng, WU Wen-Yan, XU
An-Long*
( Biochemistry Department, The Opening Laboratory for Marine Functional
Genomics Research, Zhongshan University, Guangzhou 510275, China
)
Abstract Three
full-length cDNA from Lapemis hardwickii Gray, (namely PLA2-8,PLA2-9
and PLA2-384), encoding phospholipase A2(PLA2),
were isolated and sequenced. These cDNA sequences were composed of 456 bp, 438
bp and 438 bp, encoding a signal peptide of 27 amino acids and a mature peptide
of 125, 119 and 119 amino acids, respectively. The analysis results by computer
indicated PLA2-8, PLA2-9and PLA2-384 has a pI
of ca. 4.8, 7.8 and 8.4, respectively. Sequence analysis of amino acid and
prediction of secondary structure suggested that these isoforms of PLA2
belong to class I PLA2 family. PLA2-8 was highly
homologous (81%) to Notechis scutatus scutatus (Australian tiger
snake) PLA2, PLA2-9 and PLA2-384 showed fairly
high sequence homology (90%) to Enhydrina schistosa (beaked sea snake)
PLA2, indicating that they might have similar functions. These results
reflect the diversity of Lapemis hardwickii Gray PLA2
genes. The successful cloning of these isoenzyme genes of sea snake PLA2
may provide new information for the study on structure function relationship of
PLA2 family and its possible molecular mechanism.
Key words Lapemis hardwickii Gray； phospholipase A2； sequence analysis； class I；diversity
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