Phorbol Ester Induces
Formation of A New Phospholipase D Product
CHAI Min Qiang, CHEN Jun Song, SONG Jian Guo*
( State Key Laboratory of Molecular Biology, Institute of Biochemistry and
Cell Biology, Shanghai Institutes for Biological Sciences, the Chinese Academy
of Sciences, Shanghai 200031, China )
Abstract It is
demonstrated here that phorbol-12-myristate-13-acetate (PMA) induced the
formation of a butanolated sphingolipids in A-549 cells. In cells labeled with ［3H］-serine, the distribution of
the radioactivity was easily found inphosphatidylcholine (PC),
phosphatidylserine (PS) and phosphatidylethanolamine (PE), but not in
phosphatidic acid (PA) and its immediate metabolic derivatives,suggesting that
the newly detected PLD products were derived from sphingolipids and were
different from the conventional product of PLD. Protein kinase C
(PKC)inhibitors or PKC down-regulation by prolonged PMA treatment inhibited the
PMA-induced generation of this butanolated sphingolipid, indicating that PKC
activation was required in this process.
Key words phospholipase D； protein kinase C； sphingolipid； sphingomyelinase
*Corresponding author： Tel/Fax, 86-21-64317660； e-mail, [email protected]