Expression of LIM
Protein KyoT in Adult Mouse Testis
LI Rong, HUANG Wei-Quan1, WANG Ji-Shu, SUN Qiang,
WANG Jian, ZHOU Peng, HAN Hua*
( Department of Genetics and Developmental Biology, 1Department
of Histology and Embryology, The Fourth Military Medical University, Xi’an 710032,
China )
Abstract The KyoT
expression in the adult mouse was reported here for further investigation on
the functions of KyoT in adult mouse. To study the expression of mRNA and
protein of KyoT, Northern blot, RT PCR, Immunohistochemical SABC methods and in
situ hybridization methods were used in the experiments. Two kinds of KyoT
were expressed at high levels in testis of adult mouse, and KyoT immunore
activity was mainly located in Leydig’s cells. The reactive substance was
distributed in cytoplasm rather than in nuclei. KyoT mRNA hybridization signals
were also detected in cytoplasm of Leydig’s cells rather than in nuclei. The
spermatogenic cells and negative controls showed negative results. These
results suggest that KyoT was expressd in testis of adult mouse and mainly
located in Leydig’s cells.
Key words KyoT； Leydig’s cell； testis； mouse
*Corresponding author： Tel, 86-29-3374490； e-mail, [email protected]