Expression of HIV-2 gp 105 Gene and
Characterization of gp105 in Methyltrophic Pichia
pastoris
ZHANG Ying-Jiu2*, JIN Ning-Yi1, WANG
Hong-Wei1, JIN Hong-Tao1, SHEN Jia-Cong2
( 1the Quantermarster University of PLA, Changchun 130062,
China；2Colloge of Life Science, Jilin
University, Changchun 130023,
China )
Abstract To explore a new
approach to express HIV-2 external glycoprotein gp105 in methyltrophic yeast, Pichia
pastoris, the gp 105 gene was obtained by PCR amplification and
was cloned into the secreted expression vector pHILS1. The positive recombinant
Pichia pastoris strains were screened and identified by PCR after
electroporation and induction by methanol of the gp105 expression. SDS-PAGE and
Western blot analyses showed that gp105, unlike gp120 from HIV-1, could be
secreted into medium by recombinant Pichia pastoris strain, and the
expressed product of a 90 kD glycoprotein showed satisfactory antigenicity with
specific antibody.
Key words HIV-2Rod；gp105；Pichia pastoris；expression
*Corresponding author： Tel, 86-431-8922331-3762； Fax, 86-431-8923907； e-mail, [email protected]