Synthetic Osteogenic
Growth Peptide Stimulates Osteoblast Osteogenic Activity and Enhances Fracture Healing in Rabbits
FEI Qin-Ming*, CUI Da-Fu1, CHEN
Tong-Yi, CHEN Zhong-Wei
( Department of Orthopaedics, Zhongshan Hospital, Fudan University,
Shanghai 200032； �┆ �1Institute of Biochemistry and Cell
Biology,Shanghai Institutes for Biological Sciences, the Chinese Academy of
Sciences, Shanghai 200031,
China )
Abstract sOGP was synthesized
by standard solid phase method, with the purity of 99.2% shown by HPLC and CE.
Its amino acid sequence and MS were consistent with theoretical values. In New
Zealand white rabbits, sOGP could promote the for mation of new bone and
up regulate the serum level of ALP and BGP as observed by means of biochemical
analysis, X-ray, bone mineral density, callus tissue histological analysis and
biomechanical tests, demonstrating that sOGP might play a significant role in
the tibia fracture healing. Particularly the bone density at 4 weeks and the
amounts of trabecular bone in the callus at 2 and 4 weeks of the sOGP-treated
group were significantly different from those of control group (P＜0.05). Effects of sOGP in
different medium in vitro has been studied. sOGP could accelerate
osteogenic cell proliferation in 1%BSA-10%NCS-MEM in a dose dependent manner,
with a peak at 10-11 mol/L. But interestingly, this prolife ration
activity was not observed in 10%NCS-MEM or 4%BSA-serum free MEM. It suggests
that sOGP’s osteogenic cell proliferation effects may depend on the involvement
of BSA and some regulatory molecules in serum.
Key words synthetic osteogenic growth
peptide (sOGP)； tibia fracture healing of rabbit； calvaria bone cell； BSA and serum
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