Antisense c-erbB-2 Decreases
DNA Repair and Apoptosis-Inducibility in Human Diploid Fibroblasts
ZHANG Mao, ZHANG Zong-Yu, TONG Tan-Jun*
( Department of Biochemistry and Molecular Biology, Peking University
Health Science Center, Beijing 100083, China )
Abstract pDOR-erbB-2
sense and antisense retroviral expression vectors were introduced into normal
human diploid fibroblasts(2BS), respectively, to construct 2BS-A
cells(transfected with antisense recombinant vector) and 2BS-S cells
(transfected with sense recombinant vector). Southern blot analysis verified
that the exgenous c-erbB-2 cDNA were integrated into genomic DNA.
Compared with the cells transfected with pDOR-neo empty vector, 2BS-A cells
exhibited decreased ability not only to repair DNA damage, but also to undergo
apoptosis. This was consistent with our observations that senescent phenotype
was earlier appeared in these cells.
Key words proto-oncogene； senescence； apoptosis； DNA repair
*Corresponding author： Tel, 86-10-62091454； Fax, 86-10-62015582； e-mail, [email protected]