Effect of Guanidinium
Chloride Concentration on the Renaturation of Denatured Lysozyme
SHI Jin-Hui, DONG Xiao-Yan*, SUN Yan
( Department of Biochemical Engineering, Tianjin University, Tianjin 300072,
China )
Abstract The effect
of guanidinium chloride (GdmCl) on the renaturation of denatured-reduced
chicken egg white lysozyme was studied. It was found that the renaturation
yield was significantly related to the GdmCl concentration, that is, the GdmCl
concentration required to obtain a high renaturation yield increased with the
increase of the denatured lysozyme concentration. When denatured lysozyme
concentrations were lower than 0.21 g/L, the presence of 0.7 mol/L GdmCl
resulted in the complete renaturation of lysozyme, while higher GdmCl
concentration, i.e., 1.0–1.5 mol/L, was needed to yield more than 95%
renaturation of 0.6–1.05 g/L of the denatured lysozyme. In addition, lysozyme
renaturation rate decreased with increasing GdmCl concentration. Therefore, it
is considered that optimizing GdmCl concentration is the key step to enhance
the renaturation yield of denatured proteins.
Key words lysozyme；guanidinium chloride；denaturation；renaturation
*Corresponding author：Tel,86-22-27401764；Fax，86-22-87893309； e-mail, [email protected]