Molecular Cloning and
Characterization of Rice Pollen Profilin
YE Qiu, XU Yin, YAN Fang, TANG Lin, CHEN Fang*
( The College of Life Science, Sichuan University, Chengdu 610064,
China )
Abstract Profilin is
a low molecular weight protein and is identified as an actin binding protein in
higher plants. Two pollen-specific cDNA clones of rice, RproA and RproB,
were isolated from a cDNA library of rice mature pollen. The two cDNA clones
was 821 bp and 805 bp long and contained an open reading frame of 131 amino
acids, 5′ and 3′ untranslated regions and a long poly(A) tail. The comparison
of deduced amino acid sequences from RproA and RproB cDNA and
other plant profilin cDNA indicated that the two rice profilin are high
homologous to each other, showed that 89% similarity to maize (ZmPRO3),
87% to C.dactylon, 83% to H.brasiliensis and 89% to P.pratense.
Genomic southernblot analysis suggested the existence of two genes at least.
Northern blot and reverse transcriptase polymerase chain reaction analysis
demonstrated that the two rice profilin cDNA are anther or pollen specific.
Key words actin cytoskeleton； profilin； cloning and expression； pollen
*Corresponding author： Tel, 86-28-5417281； Fax, 86-28-5417281； e-mail, [email protected]