Cloning and Sequencing
of the Genome Segment S9 of Rice Black-streaked Dwarf Virus
ZHANG Heng-Mu1,2, CHEN Jian-Ping1*,
CHENG Ye1, LEI Juan-Li1, XUE Qing-Zhong2
( 1Virology Laboratory, Zhejiang Academy of Agricultural
Sciences, Hangzhou 310021, China；2College of Aagriculture and
Biotechology, Zhejiang University, Hangzhou 310029, China )
Abstract Genome
segment S9’s of rice black streaked dwarf virus (RBSDV) of three Chinese
isolates were amplified by RT-PCR and sequenced,and were found to be consisted
of 1 900 nt(RBSDV-Zj S9 EMBL accession number：AJ297430),1 898 nt (RBSDV-Heb
S9 EMBL accession number： AJ297429) and 1 900 nt (RBSDV-Hub S9
EMBL accession number：AJ291706), respectively. Genome segment
S9’s of three Chinese Isolates shared 98.5%–98.8% sequence homology and all
contained two open reading frames (ORF), which encoded two polypeptides with
moleclular weights of 40 kD and 24 kD, respectively. Amino acid sequence
comparison of the polypeptides encoded by the second ORF of the corresponding
genomic segment of five isolates, including three Chinese isolates, Japanese
isolate, and Italian MRDV (maize rough dwarf virus), were highly conserved.
Key words RBSDV；genome segment S9；sequence analysis
*Corresponding author： Tel, 86-571-6404242； Fax, 86-571-6404258； e-mail, [email protected]