New Frontiers in the
Proteome Research： Quantitative
Proteomics
YUAN Quan, ZHAO Fu-Kun*
( Institute of Biochemistry and Cell Biology, Shanghai Institute for
Biological Sciences, the Chinese Academy of Sciences, Shanghai 200031,
China )
Abstract In the past
years, proteome research has achieved very exciting advances. The automation of
2DE-MS approach and the use of multidimensional liquid chromatography (LC) and
tandem mass spectrometry (MS/MS) have overcome many disadvantages of
two-dimensional gel electrophoresis. The stable isotope labeling and the
isotope-coded affinity tags (ICAT) strategy provided an ideal method for
accurately quantitating the low copy number proteins which play a key role in
cells or tissues. At the same time, the research of the quantitative proteomics
has been improved greatly with the development of the protein chip technology.
Recent advances of the research in the quantitative proteomics and the related
technologies are reviewed.
Key words quantitative proteomics； two-dimensional gel
electrophoresis； molecular scanner； isotope-coded affinity tags
(ICAT)；
protein chip
*Corresponding author： Tel, 86-21-64374430-5245； e-mail, [email protected]