Effect on Glucose
Transport and Anion Exchange in Human Erythrocytes by Mechanical Force Factors
LI Yue-Zhou, ZHOU Han-Qing, PENG Feng, ZHANG Zhi-Hong*
( Liren Laboratory, Department of Physiology and Biophysics, School of Life
Sciences, Fudan University, Shanghai 200433, China )
Abstract The
mechanical forces play an important role in both the normal physiology and the
pathology of the cardiovascular system. It was observed that an increase in
speed of rotary flow of the intact erythrocyte suspension resulted in increase
in the rate of glucose entry across the erythrocyte membranes. The effects of
osmotic stress and a membrane curvature altering drug, chlorpromazine, on the
glucose and anion transport were also shown. The decrease in the activation
energy for glucose and anion transport according to Arrhenius plot for
temperature dependence of membrane transport meant that thermodynamic barrier
for transporter reorientation in the membranes was reduced. The data from the
measurement of intrinsic fluorescence quench in ghosts indicated that the
conformations of both glucose transport protein (GLUT1) and anion transport protein
(band 3) in the erythrocyte membranes were affected by the mechanical force
factors. After inhibition of anion transport, a modification of the response of
glucose transport by the mechanical force was observed, which also indicates
that there is an information linkage between GLUT1 and band 3 in the
erythrocyte membranes.
Key words mechanical force； erythrocytes； glucose transport；anion exchange
*Corresponding author： Tel, 86-21-65643673； Fax, 86-21-65650149； e-mail, [email protected]