Effects to HeLa Cells of
the Inhibition of Survivin by Antisense RNA
MEI Zhu-Zhong, DONG Yan, SUN Zhi-Xian*
( Institute of Radiation Medicine, Academy of Military Medical Sciences,
Beijing 100850, China )
Abstract To study
the function of survivin in cancer cells, survivin cDNA was amplified from HeLa
cells by RT-PCR. Then the coding fragment was subcloned into an inducible
eukaryotic expression plasmid pHC in reverse direction.The obtained plasmid
pHSC was transfected into HeLa cells by Lipofectamine. An inducible cell line
which can express antisense RNA of survivin with 2 mmol/L Zn2＋ was obtained by the selection
with 800 nmol/L G418 solution. The expression of antisense RNA of survivin
inhibited the expression of survivin protein. Inhibition of survivin expression
suppressed the proliferation of HeLa cells and its cell cycle.And it also
sensitized HeLa cells to chemotherapeutics.
Key words survivin； antisense RNA； cancer
*Corresponding author： Tel, 86-10-66932212； e-mail, [email protected]