Effects of the
Superoxide Anionic Radicals to the Cultured Cerebral�–ortex Neuron of Newborn
Rat
JIANG Zhao-Feng*
( Department of Biochemistry, College of Arts and Sciences, Beijing Union
University, Beijing 100083, China )
HE Xiang-Ping, LIU Chuan-Hui
( Institute of Poison and Drug, Academy of Military Medical Sciences,
Beijing 100850, China )
YANG Han-Yi
( Department of Biochemistry, Medical School, Jilin University, Changchun 130021,
China )
Abstract The effect
of the superoxide anionic radicals to the cultured cerebral cortex neuron of
newborn rat was investigated by using xanthine oxidase/xanthine. The results
are as follows. The growth of neuron was inhibited； ATPase activity decreased to
4.223 μmol/(mg・min-1)(Pr)； total cellular carbonyl
increased to 295.40 μmol/g wet weight； lipid peroxides(LPO) increased
to 4.87 nmol/g(Pr)； membrane lipid fluidity decreased (P
value：
0.398)；
in nuclear DNA, thesingle strand breaks(SSB) increased ［in the single cell gel
assay(SCG), DNA migration was 7.35 mm］； subcellular structures of the
neuron changed (mitochondria were swelling, etc.)； and SOD gene expression of
the neuron increased (the SOD activity and the SOD content increased, SOD mRNA
content increased). These suggest that the superoxide anionic radicals could
damage proteins, nucleic acids, lipids and subcellular structures in neurons.
Key words superoxide anion； neuron damage； superoxide dismutase； lipid； nucleic acid
*Corresponding author： Tel, 86-10-62004522； Fax, 86-10-62388927； e-mail, [email protected]