Expression of Exogenous
E-cadherin Regulates Anchorage-independent Growth in Human Lung Adenocarcinoma
Cells
HUANG Chuan-Xin, CHEN She, YING Miao, SHEN Zong-Hou*
( Department of Biochemistry, Medical Center of Fudan University, Shanghai 200032,
China )
Abstract To explore
the suppressive effects of E-cadherin on aggregation and anchorage-independent
growth of human lung adenocarcinoma cells, a mammalian expression vector
containing full E-cadherin cDNA was constructed and transfected into a
metastatic human lung adenocarcinoma cell line A549. RT-PCR and Western blot
were used to analyse expression levels of E-cadherin. Cells aggregation and
anchorage-independent growth of the tumor cells before and after gene
transfection were assessed respectively. The results indicated that stable
transfectants showed markedly increased levels of expressed E-cadherin compared
with the corresponding sham transfectants. The transfections showed more
intense cellular aggregation and anchorage independent growth.
Key words E-cadherin； cell aggregation； anchorage independent growth
*Corresponding author： Tel, 86-21-64041900-2299； Fax, 86-21-64039987； e-mail, [email protected]