Cloning and Distribution
of rRP58, A Novel Neuronal
Gene
KANG Jian-Sheng1, YANG Yong-Chen, LIU Hai-Lan, LI
Yong-Hong, DU Yu-Cang1, LI Rong-Xiu*
( Research Center of Biotechnology, Shanghai Institutes for Biological
Sciences,the Chinese Academy of Sciences, Shanghai 200233, China； 1Institute of Biochemistry and Cell
Biology, Shanghai Institutes for Biological Sciences, the Chinese Academy of
Sciences, Shanghai 200031,
China )
Abstract EST
(AW055733) is a 3′-cDNA fragment specially expressed in rat brain. It is
homologous to human and mouse RP58 gene, which is a transcriptional
repressor gene. Primers were designed based on these two genes, and then two
transcripts of rRP58 gene were got from male SD rat using RT-PCR
method. The rRP58 protein is a C2H2 type zinc finger
protein, containing POZ domain at N-terminal and zinc finger domain (ZFD) at
C-terminal. In addition, there is a highly acidic region between POZ and ZFD.
POZ and ZFD were cloned, expressed and purified. Then, the antibodies to POZ
and ZFD were prepared, and used to analyze distribution of alternative
transcripts of rRP58.
Key words rRP58； zinc finger domain； POZ； rat brain； antibody
*Corresponding author： Tel, 86-21-64700892-356； Fax, 86-21-64700244； e-mail, [email protected]