Preliminary
Crystallographic Analysis of Phospholipase A2 from Naja naja
kaouthia Lesson Venom
WANG Zhi-Qing, ZHUANG Mao-Xin, SONG Shi-Ying1, LIN
Zheng-Jiong1*, SHU Yu-Yan*
( Snake Venom Research Institute, Guangxi Medical University, Nanning 530021,
China；1National Laboratory of Biomacromolecules,
Institute of Biophysics, the Chinese Academy of Sciences, Beijing 100101, China )
Abstract An acidic
phospholipase A2 isolated from the venom of Naja naja kaouthia
Lesson in Guangxi exhibits anticoagulative and hemolytic activities. In this
work, the enzyme was crystallized by the method of hanging drop vapor
diffusion. Two crystal forms were obtained and characterized by X-ray diffraction.
One of them belonged to space group P43212 or P41212
with unit cell parameters a＝b＝8.797 nm, c＝10.831 nm and there were three
molecules per asymmetric unit； the other belonged to space group P213
with unit cell parameters a＝b＝c＝6.840 nm and there was one
molecule per asymmetric unit.The diffraction data were collected up to 0.28 nm
for each crystal form. The crystal properties of Naja naja verom
phospholipase A2 from different geographical regions are compared.
Key
words
cobra venom；phospholipase A2；crystallization
*Corresponding authors：SHU Yu-Yan：Tel,86-771-5353495；Fax,86-771-5353495；e-mail, [email protected]；LIN Zheng-Jiong：Tel,86-10-64888513；Fax,86-10-64877837；e-mail, [email protected]