Sequence Analysis of the
BamHI-J Fragment of the Spodoptera
litura Multicapsid Nucleopolyhedrovirus
WANG Li-Hua, YU Jian-Xiu, HU Xiao-Hui, PANG Yi*
( State Key Laboratory for Biocontrol and Institute of Entomology,
Zhongshan University, Guangzhou 510275, China )
Abstract The BamHI-J
fragment located at 25.8–9.9 map units of the Spodoptera litura
multicapsid nucleopolyhedrovirus (SpltMNPV) genome was sequenced. The fragment
contained four ORFs, one partial ORF potentially encoding C-terminal of
chitinase gene and one partial homologous region (hr). The four ORFs
included lef-8 gene, J domain protein gene (bjdp gene),
ORF570 and ORF165. The ORF570 revealed 31% identity to the helicase-2 of Lymantria
dispar MNPV. The ORF165 was unique to the SpltMNPV. The bjdp
gene, reported here for the first time in baculoviruses, was one of J domain
family protein genes, and the predicated amino acid sequence possessed a
characteristic of J domain protein of other DnaJ proteins at its N-terminus.
The lef-8 showed high identities to the homologs of reported
baculovirus genomes. As a component of virus-encoded RNA polymerase, the LEF-8
of SpltMNPV had the conserved motif GIKICGIHGQKG near the C-terminal end.
Analysis of the LEF-8 phylogenic tree demonstrated SpltMNPV was very closely
related to SpliMNPV.
Key words Spodoptera litura
multicapsid nucleopolyhedrovirus；
lef-8 gene； J domain protein； sequence analysis
*Corresponding author： Tel, 86-20-84113860； Fax, 86-20-84037472； e-mail, [email protected]