Isolation and
Characterization of EDAG-1, A Novel
Gene Related to Regulation in Hematopoietic System
LÜ Jun2, XU
Wang-Xiang1, WANG Si-Ying3, ZHAN Yi-Qun, JIANG Yan1,
CAI Wei-Min2, YANG Xiao-Ming1*
(1Institute of Radiation Medicine, Academy of Military Medical
Sciences, Beijing 100850, China； 2Institute of Infectious
Disease, First Affiliated Hospital, Medical School, Zhejiang University,
Hangzhou 710006,
China； 3Department of Pathology, Anhui Medical
University, Hefei 230000,
China )
Abstract A novel
gene, named embryonic develop associated gene 1 (EDAG-1) and
abundantly expressed in human fetal liver tissues, was isolated by screening a
human fetal liver cDNA library and the 5′RACE. The full length of EDAG-1
mRNA is 2 166 bp, with an open reading frame of 1 452 bp neucleotides, encoding
a 484 amino acid protein. No domain or motif was found similar with other genes
by Blast program. Two copies of AUUUA motif in 3′non-translated region show
instability of its mRNA. The molecular weight of the protein is 55.3 kD
identified by the translation in vitro. EDAG-1 is
specifically expressed in hematopoietic tissues, and is quickly down-regulated
during the differentiation of K562 cells induced by hemin and EPO. These
results show that EDAG-1 is related to the regulation in hematopoietic
system and the development of leukemia.
Key words EDAG-1； K562 cells； differentiation； regulation in hematopoietic
system
*Corresponding author： Tel, 86-10-66931424； Fax, 86-10-68214653； e-mail, [email protected]