Expression of the
Recombinant Human Interleukin-11 in Pichia pastoris
WANG Tong-Ying, WANG Chang-Mei*, WEI Guang, QIU Ji-Wan,
HUANG Yan-Shan
( Research Department of Jiuyuan Gene Engineering Co. LTD, Hangzhou 310018,
China )
Abstract Full length
cDNA of human IL-11 was synthesized by DNA synthesizer. An expression
plasmid, pGENYk, containing the recombinant DNA fragment, was linearized and
transformed into Pichia pastoris. This recombinant gene was highly
expressed in this yeast, and the expression product was purified by a
three-step chromatography method. Analysis of the purified recombination
protein with SDS-PAGE, Western blot and biological activities showed that the
activity of the protein was the same as the Neumega expressed in E.coli.
Key words rhIL-11； Pichia pastoris； expression
*Corresponding author： Tel, 86-571-86910099-201； e-mail, [email protected]