Superimposition and
Analysis of ESI Tandem Mass Spectra
WANG Xian-Chun, LIANG Song-Ping*
( College of Life Science, Hunan Normal University, Changsha 410081,
China )
Abstract Methionine
enkephalin and four synthetic peptides (a heptapeptide and its modified form, a
decapeptide and an icosapeptide) were fragmented using ESI tandem mass
spectrometry with certain varying instrumental parameters, such as amplitude of
excitation, to produce a series of MS/MS spectra. Using the Bruker DataAnalysis
software, some complementary MS/MS spectra were chosen to form a superimposed
MS/MS spectrum with consecutive triplets (as well as a few doublets)consisting
of product ions AnBnCn or XnYnZn.
The amino acid sequences of the peptides were easily and accurately determined
from the superimposed MS/MS spectrum, suggesting that the method is easy and
suitable for peptide sequencing by tandem mass spectrometry.
Key words tandem mass spectrometry； sequencing； spectrum superimposition； peptide
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