Identification of A
Protein Interacting with Type 2 Methionine Aminopeptidase by Yeast Two-hybrid
System
LIU Wei-Feng*, LIU Jun1
( State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100,
China； 1Center for Cancer Research, Massachusetts
Institute of Technology, MA
02139, USA )
Abstract Type 2
methionine aminopeptidase (MetAP2) is the molecular target for the fumagillin
inhibitors against angiogenesis. Making use of the yeast two-hybrid system with
GAL4 DBD-fused MetAP2 as a bait, a human brain cDNA library was screened to
isolate protein factors that might interact with MetAP2. Among the 2×106
transformants obtained, five positive clones were picked out. Sequence analysis
revealed that three of them contained cDNA fragments from flotillin and encode
a carboxy terminus (starting from amino acids 145–233, respectively) of
flotillin protein. The interaction between MetAP2 and flotillin detected by
yeast two-hybrid system suggests that MetAP2 might play a role in some
biological processes where flotillin is involved.
Key words yeast two-hybrid system； type 2 methionine
aminopeptidase； flotillin； angiogenesis
*Corresponding author： Tel, 86-531-8564431； Fax, 86-531-8565234； e-mail, [email protected]