Cloning and
Sequence Analysis of Full-length Trypsin cDNA of Culex pipiens pallens
SHEN Bo, TIAN Hai-Sheng, Ma Lei, LI Xiu-Lan,
ZHEN Shi-Zhong, ZHOU Da-Hu, ZHU Chang-Liang*
( Parasitology Department of
Nanjing Medical University, Nanjing 210029, China )
Abstract The
expression difference of trypsin gene between deltamethrin-resisitant strain
and susceptible strain of Culex
pipiens pallens was further investigated, and the results showed that the expression of trypsin gene
were respectively 4.3 and 3.9 fold more in the resistant strain than in the
susceptible strain, by reverse
Northern blot and Northern blot assays. A full-length trypsin cDNA of 909 base
pairs (GenBank/NCBI AY034060) with an open reading frame of 786 base pairs was
cloned from the constructed cDNA library by the rapid amplification of cDNA
ends, and the deduced protein of
261 amino acids was 55% homologous with Anopheles gambiae trypsin.
Key words Culex pipiens pallens; pesticide resistance; trypsin gene； reverse Northern blot;
Northern blot
*Corresponding author: Tel, 86-25-6663285; e-mail, [email protected]