Effects of
Blastocyst Surface Oligosaccharide LeY on Secretion and Expression
of Matrix Metalloproteinase in vivo
GE Chang-Hui, KONG Ying, WANG
Han, ZHU Zheng-Mei*
( Department of Biochemistry,Institute of Glycobiology, Dalian Medical
University, Dalian 116027, China )
Abstract The effects
of oligosaccharide LeY on expression and secretion of matrix metalloproteinase
(MMP) and tissue inhibitor of metalloproteinase 1 (TIMP1) of embryos were
investigated in cultured mouse blastocysts by zymography, immuno-dot blots and
RT-PCR after blocking the oligosaccharide on embryo surface with specific
monoclonal antibody AH6. The cultured blastosycts were treated with antibody
AH6 for 3 h, then the antibody was removed and culture was continued, and the
expression and secretion of MMP and TIMP1 were analyzed at 1.5 h, 3 h and 6h.
It was shown that, only 1.5 h after blocking, the expression of MMP2
gene and MMP9 gene in embryo was significantly declined, and expression
of TIMP1 was slight increased, then secretions of MMP2, MMP9 were also
declined at 3 h, but secretion of TIMP1 had no significant change during the period
of observation. The results indicate that LeY may play a role of
regulating the expression and secretion of MMP of mouse embryo at
pre-implantation stage, by controlling the expression of MMP2 gene and MMP9
gene.
Key words embryo; LeY oligosaccharide
antigen; matrix metalloproteinase; monoclonal antibody
*Corresponding author: Tel,
86-411-4720616; e-mail, [email protected]