In vitro
Transcription of Inducible nod Gene nodA
ZHANG Lei, HONG Guo-Fan
( Shanghai Institute of Biochemistry, the Chinese Academy of Sciences,
Shanghai 200031, China )
Abstract We observed
in vitro transcription of nodA, an inducible nod gene.
Primer extension analysis of the transcripts showed there always existed a
transcription product identical to that of its in vivo transcription,
when 10 μmol/L naringening and NodD protein was added in the in vitro
transcription system. Induced in vitro transcription of nodA gene
suggests that in its in vivo transcription there may also exist a kind
of physical interaction among NodD protein, inducer naringening and promoter of
nodA gene.
Key words inducible nod gene; in vitro
transcription; induce
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