Overexpression
of EDAG-1 in NIH3T3 Cells Leads to Malignant Transformation
Lü Jun, XU Wang-Xiang1, WANG
Si-Ying1, JIANG Yan1, LI Chang-Yan1, CAI
Wei-Min, YANG Xiao-Ming1*
( Institute of Infectious Disease, First Affiliated Hospital, Medical
College of Zhejiang University, Hangzhou 710006, China; 1Institute
of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850,
China )
Abstract To study
the biological function of EDAG-1, a novel gene cloned by our lab
recently, the EDAG-1 cDNA was subcloned into pcDNA3.1(+)vector and
transfected into NIH3T3 cells by Lipofect AMINE methods. Overexpression of EDAG-1
in NIH3T3 cells resulted in loss of cell contact inhibition,
anchorage-independent growth in soft agar and tumor formation in nude mice.
These results suggest that EDAG-1 is a transforming gene and may be
associated with the development of tumors, especially in hematopoietic system.
Key words EDAG-1; gene expression;
transformation
*Corresponding author: Tel,
86-10-66931424; Fax, 86-10-68214653; e-mail,[email protected]