Identification
and Tissue-specific Expression of A Full Length cDNA Encoding ZIP Protein in Arabidopsis
ZHENG Cheng-Chao*, YANG
Guo-Dong, WANG Dan, LI Hong-Mei
( College of Life Sciences, Shandong Agricultural University, Taian 271018,
China )
Abstract A
full-length cDNA encoding a putative ZIP proteins, has been isolated from Arabidopsis
cDNA library using a heterologous DNA probe. Sequence analysis showed that the
deduced protein contained a leucine zipper and a possible nuclear localization
signal domain. There was no identical proteins in the database we searched, but
it shared high sequence homology with PNZIP from Pharbitis nil and two
other open reading frames (ORF) of unknown function from Synechocystis
spp and Porphyra purpurea. These together defined a new family of
evolutionarily conserved leucine zipper proteins, which might play a role as a transcription
factor in the regulation of gene expression. Southern and Northern blot
analysis indicated that AT103 was a single copy gene and the
accumulation of mRNA was obviously different in leaves, stems and roots in Arabidopsis.
Key words cDNA clone; leucine zipper; transcription
factor; Arabidopsis
*Corresponding author:
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