Mouse Restin
Inhibits Bovine Aortic Endothelial Cell
Proliferation and Causes Cell Apoptosis
XU Ren, XIN Li, FAN Yi1,
MENG Hai-Rong, LI
Zai-Ping, GAN Ren-Bao*
( Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, the Chinese Academy of Science, Shanghai 200031, China; 1Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China )
Abstract
Restin, a homologous protein of
endostatin, was found by
Ramchandran et al. It was the C terminal fragment of type XV collagen.
To analysis the inhibition activity of mouse restin on the proliferation of
endothelial cells, the cDNA of restin was amplified from the total RNA of the
mouse muscle and cloned into the prokaryotic expression plasmid pQE32. The
recombinant protein was expressed in inclusion body with a yield about 60%―70%
of total protein. After refolding, the purified recombinant protein
specifically inhibits bovine aortic endothelial (BAE) cell proliferation
stimulated by basic fibroblast growth factor (bFGF) in a dose dependent manner,
but the activity of restin was weaker than that of endostatin. Treatment of BAE
cell with recombinant restin caused G1 arrest and apoptosis in BAE
cells.
Key words restin; endostatin; collagen XV;
angiogenesis inhibitor; apoptosis
*Corresponding author: Tel, 86-21-64374430-5325; Fax, 86-21-64338537;
e-mail, [email protected]