Cloning and
Expression of Human Calcyclin Binding Protein (hCacyBP) Gene
LIU Wei-Xia, WU Jing, ZHAO
Zhao, ZHOU Yan, PENG Xiao-Zhong, YUAN Jian-Gang*, QIANG Bo-Qin*
( Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union
Medical College, Beijing 10005, China )
Abstract Human
calcyclin binding protein (hCacyBP) gene was obtained by the screening of a
human cDNA library. The full coding region of CacyBP was cloned into E.coli
strain pET28, and then was expressed and purified through affinity
chromatography. Rabbit anti-human CacyBP polyclonal antibody was obtained by
immunizing rabbit with the purified human CacyBP. Western blots showed that it
was expressed extensively in many tissues of mouse. The results of
immunohistochemistrial staining showed that the location of CacyBP in BT325
cell line before and after differentiation changed from cytoplasm into nucleus
and perinucleus cytoplasm.
Key words calcyclin; calcyclin binding protein
(CacyBP); cloning; expression
*Corresponding author:
Tel, 86-10-65296411; Fax, 86-10-65240529; e-mail, [email protected]