Preparation, Characterization and Receptor-binding
Capacity of Pig Serum Transferrin Half-molecules Containing a Single Iron-binding
Site
ZHANG Xin-Tang, TANG Yue-Hua, FENG
You-Min*
( State Key Laboratory of Molecular Biology, Shanghai Institute of
Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, the
Chinese Academy of Sciences, Shanghai 200031, China )
Abstract N- and
C-half molecules containing a single iron-binding site were simultaneously
obtained from trypsin digest of iron-saturated pig transferrin. The activities
of the pig serum transferrin and of its N- and C-half molecules to bind the human
placental membrane transferrin receptor were compared.The results indicate that
the receptor-binding site of pig transferrin may be located at the C-half
molecule of the transferrin.
Key words transferrin; half-molecule; receptor-binding
*Corresponding authot:
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86-21-64338357; e-mail, [email protected]