Gene
Expression Pattern of Senescence Associated Genes in Rat Muscle
ZHANG Fang-Lin*1,2, HUANG
Guo-Liang2, YE Chuan-Zhong3, ZHANG Kai-Zhen2,
LIN Zhe-Zhang2, CHEN Xiao-Chun4, YOU Jie2
( 1Shanghai Second Medical University, Ruijin Hospital, Shanghai
Institute of Endocrinology, Shanghai 200025, China; 2Fujian
Union Hospital Affiliated to Fujian Medical University, Fujian Institute of
Endocrinology, Fuzhou 350001, China; 3Fujian Union
Hospital Affiliated to Fujian Medical University, Urology Department, Fuzhou 350001,
China; 4Fujian Institute of Geriatrics, Fuzhou 350001,
China )
Abstract This work
is aimed at the investigation of the senescence associated genes differentially
expressed in rat muscle by cDNA microarray. After total RNA isolation, mRNA
purification and probe preparation, the gene expression patterns of rat muscle
from the old group (30 months old) and the young group (3 months old) were
compared by cDNA gene chips. In the muscle of old rats there were reproducibly
127 differentially expressed genes, among which some down-regulated genes were involved
in energy metabolism and signal transduction, while some up-regulated genes
were related to protein degradation and cell apoptosis. Meanwhile muscle fiber
atrophy with mitochondria vacuolation was found in aging rat by electron
microscope.
Key words gene chip; gene expression pattern;
senescence; skeletal muscle
*Corresponding author： Tel, 86-21-63050615；e-mail： [email protected]