Quantification
of Metallothionein Induced with Selenium by Using Competitive ELISA
LIU Ying, JIANG Guan-Qun, RU Bing-Gen*,
XU Zhuo-Li1, GUO Jun-Hua2
( National Laboratory of Protein Engineering, College of Life
Sciences, Peking University, Beijing 100871, China; 1Military
Medical College, Beijing 100071, China; 2Department of
Clinical Pharmacology, North Taiping Road Hospital, Beijing 100039,
China )
Abstract
Metallothionein induced by selenium was assayed with competitive ELISA. It was
found that in control mice, the liver contained (2.47±0.90) μg MT/g wet weight
tissue, while the mice administered with ZnSO4 contained
(8.15±2.20)μg MT/g wet weight liver tissue and the mice given with selenium
malt contained (12.80±1.44) μg MT/g wet weight liver. A significant difference
was found between the control and the latter two groups (P<0.05, P<0.05).
The MT content of selenium group was significantly higher than the zinc group.
It is suggested that the selenium was more potent in induction of MT and was a
stronger inducer than ZnSO4 in its effective dose range, therefore
selenium may be developed into a novel and safe MT inducer.
Key words selenium; metallothionein; competitive ELISA
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86-10-62751842; Fax, 86-10-62751842; e-mail,
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