Synthesis of
Ser-His-Gly・DNA Conjugates
LI Xi-Qing, LIU Hai1, ZHUANG
Min, TAN Bo1, ZHAO Yu-Fen1, LU Chang-De*
( Institute of Biochemistry and Cell Biology, Shanghai Institutes for
Biological Sciences, the Chinese Academy of Sciences, Shanghai 200031,
China; 1The Key Laboratory of Bioorganic Phosphorus
Chemistry, Department of Chemistry, Tsinghua University, Beijing 100084,
China )
Abstract There are
strong interactions between the bases of oligonucleotides. Based on
Watson-Crick principle, they can form stable secondary and tertiary structure
such as hairpin, duplex, triplex, G-quartet, pseudoknot, which can serve as the
scaffold of molecules. Peptides contain active groups such as amino, carboxyl,
imidazole, hydroxyl. A protected Ser-His-Gly-Threoninol phosphoramidite was
synthesized in this work and was incorporated into a triplex-forming
oligonucleotide. The results indicate that the oligonucleotide containing
Ser-His-Gly-Threoninol in the middle could still form triplex with the target
duplex and the dissociation constant was 0.5 μmol/L.
Key words oligonucleotide backbone; peptide;
Ser-His-Gly; phosphoramidite
*Corresponding author：Tel, 86-21-64374430-5234; Fax,
86-21-64338357; e-mail, [email protected]