Synthesis and
Oxidative Refolding of Hainantoxin-IV
LIU Zhong-Hua, CHEN Ping, LIANG Song-Ping*
( College of Life Science of Hunan Normal University, Changsha 410081,
China )
Abstract
Hainantoxin-IV, a neutoxic peptide from the spider Selenocosimia hainana,
was synthesized by solid-phase method with fluorenylmethyoxycarbonyl amino
acids (Fmoc-AA). Reverse-phase HPLC was used to monitor the oxidative folding
of synthetic Hainantoxin-IV under different reaction conditions in order to
find optimal conditions for renaturation of synthetic Hainantoxin-IV. The best
renaturation yield was received in 5 mmol/L GSH and 0.5 mmol/L GSSG at pH 8.0
in 0.1 mol/L Tris-HCl and 0.1 mol/L NaCl buffer. The renaturated Hainantoxin-IV
was monitored with MALDI-TOF MS、reverse-phase HPLC and isolated mouse
phrenic nerve-diaphragm preparation.
Key words Hainantoxin-IV; solid-phase synthesis; oxidative
folding; biological activity
*Corresponding author：Tel, 86-731-8872556; Fax,
86-731-8641304; e-mail, [email protected]