Application
of Bio-mass Spectrometry in Cellular Signal Transduction
ZHANG Xue-Min* ， WEI Kai-Hua， YANG Song-Cheng
( National
Center of Biomedical Analysis, Academy of Millitary Medical Sciences,
Beijing 100850, China;
Institute of Basic Medical
Science, Academy of Milit
ary Medical Sciences, Beijing 100850, China )
Abstract The quickly developing
techniques of biological mass spectrometry (bio-MS) in recent years realized
the high throughput identification of proteins by determining the accurate mass
values of trypsin digested peptides and the randomly selected peptide sequence
tags, and have been successfully used in the studies of protein interactions
and post-translational modification such as the phosphorylation. Compared to
the conventional approaches, the above techniques can identify all the
phosphorylated proteins (including their phosphorylated amino acid sites)
involved in a multi-signal pathway in a single experiment, and they have been
developed into a hot-spot of proteomics. The three strategies for the
application of bio-MS in the above fields are briefly reviewed.
Key
words
bio-mass spectrometry； signal transduction； phosphorylation； proteomics； protein interaction
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