Molecular Cloning of SRG2, a Mouse Testis Spermatocyte Apoptosis-related Gene

Abstract

A
novel mouse gene full-length cDNA sequence—SRG2 were
identified (GenBank accession number AF395083), which was significantly changed
in cryptorchidism, from a mouse testis cDNA library using a cDNA fragment
(GenBank accession number BE644542) as an electronic probe. SRG2
was 1058 bp in length. The putative protein encoded by this gene was 295 amino
acids with a theoretical molecular weight of 33 579 and isoelectric point
of 9.64. The sequence shared no signi fican t homology with any known protein
in databases except TSARG2, with which its hom ology was
78%. RT-PCR showed that SRG2 was expressed significantly
in testis.