The
Binding Sites of Estrogen Receptor for Miyabenol C and Kobophenol A
TIAN Chun-Yan, GAO Hai-Dong1, ZHANG Yu-Gao, XU Guang*, HU Chang-Qi2,
SONG Hou-Yan
( Laboratory of
Molecular Genetics, Shanghai Medical School, Fudan University, Shanghai 200032,
China;
1Department of General Surgery, Qilu Hospital, Shandong University,
Jinan 250012, China;
2Department of Chemistry of Natural Drugs, School of
Pharmacy, Fudan University, Shanghai 200032, China )
Abstract
To examine the binding sites of miyabenol C ( Miy C ) and kobophenol A ( Kob A ) with
estrogen receptor ( ER ), computer modeling was applied to determine 3D structure of Miy C and Kob A.
Molecular docking of the components to ER was carried out to find the binding sites between them. PCR
mutagenesis was used to change the structure of ER cDNA. After the mutated sites were confirmed by
DNA sequencing, report gene assay was used to study the effects of Miy C and Kob A on the
trans-activating ability of ER. Results indicated that the effect of Miy C on the trans activating
ability of mutant 1 of ER [M1ER (ER M517AG521D)] was decreased, and Kob A had no
stimulating effects on the trans-activating ability of M1ER. Miy C and Kob A had no stimulating effects
on the trans activating ability of mutant 2 of ER [M2ER (ER E353GR394G)].
Therefore, the ER sites for Miy C and Kob A may be located at Glu353, Arg394,
Met517 and Gly521.
Key
words estrogen receptor; binding site; phytoestrogen
Corresponding
author: Tel,
86-21-54237440; e-mail, [email protected],
[email protected]