The
Interactions of LIM Protein KyoT with Polycomb Proteins
LI Rong, ZHANG Ru1*
( Institute of Microbiology Epidemiology, Academy of
Military Medical Science, Beijing 100071, China;
1 Neurological Department of the Second Hospital, Xi’an Jiao Tong
University, Xi’an 710004, China)
Abstract LIM domain protein KyoT interacts with
transcription factor RBP-J and modulates Notch signaling pathway. To study the
function of KyoT, yeast two-hybrid was performed and proteins interacting with
KyoT2 including Ring1 and hPc2 (human polycomb 2) that belong to PcG(polycomb
group) were analyzed. The interactions between Ring1, hPc2, and KyoT2 were
confirmed by changing vectors in two-hybrid analysis. Their interactions in
vitro were confirmed by GST-pull down assay. Two-hybrid assay showed that
the LIM domain of KyoT was responsible for interacting with the full-length
Ring1 and hPc2. Moreover, the LIM domain also interacted with the C-terminus of
hPc2. Interactions of KyoT2 with Ring1 and hPc2 suggested that PcG family might
be involved in Notch signaling pathway.
Key
words KyoT; Ring1; hPc2; yeast
two-hybrid
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