A
High-resolution Genetic and Physical Map of a Mouse Coat Abnormity Locus (Uncv)
SHI Yao-Zhou1,2,
WANG Dong-Ping3, HU Lan-Dian2, ZHAO Guo-Ping2,
WANG Yu-Zhen1, KONG Xiang-Yin2*, LI Shan-Ru3*
( 1 School of Life Science, University of Science
and Technology of China, Hefei 230027, China;
2 Research Center of Biotechnology, Shanghai Institutes for
Biological Science, the Chinese Academy of Sciences, Shanghai 200233, China;
3 Institute of Jingfeng Medical Laboratory Animals, Beijing 100071,
China )
Abstract More than a hundred of loci (genes)
affect the development of mouse and human hair. A locus of Uncv
(uncovered) has been confirmed to be involved in hairlessness for homozygote
and sparse hair for heterozygote. Except hairlessness (or called uncovered
coat), the homozygote was also accompanied by growth retard and puberty delay.
Identification of the mutation in the gene will be important for understanding
the related diseases in human. Although the uncovered locus (Uncv) has
been mapped to the mouse distal chromosome 11(Chr11), the high-resolution
genetic map and physical map of the locus has not been created. In this study,
2074 F2 mouse populations from backcross [BALB/c (Uncv/Uncv) ´C3H (+/+)]´BALB/c (Uncv/Uncv ) and
[BALB/c (Uncv/Uncv)´C57BL/6 (+/+)]´BALB/c(Uncv/Uncv) were
genotyped using 16 polymorphic markers with an about 20 cM interval on mouse
distal Chr11. By genetic linkage analysis, Uncv locus was mapped to an about
1.4 cM interval between markers D11Mit337 and D11Mit338 with the following
order: proximal D11Mit338-D11Mit203 (Uncv)-D11Mit103 -D11Mit337 distal
on mouse Chr11. And then, a contig of 35 BACs representing the Uncv-containing
region was constructed. The contig covered 800–1000 kb region flanked by
189K10-SP6 and D11Mit103. Together, we have constructed the high-resolution
genetic map and detailed physical map of the Uncv region. This will
facilitate the identification of the Uncv loci.
Key
words mouse;
hair; mutation; genetic map; physical map
Corresponding
author:
KONG Xiang-Yin:
Tel, 86-21-64678976; Fax, 86-21-64678976; e-mail, [email protected]
LI Shan-Ru: e-mail, [email protected]