Induced
Expression of the Gene for NADP-malic Enzyme in Leaves of
Aloe vera L. under Salt Stress
SUN
Shu-Bin, SHEN Qi-Rong, WAN Jian-Min1, LIU Zhao-Pu*
( College of Resources and Environmental Science, Nanjing
210095, China;
1 Institute of Rice, Nanjing Agricultural
University, Nanjing 210095, China )
Abstract A cDNA fragment for NADP-malic enzyme,
catalyzing the reversible oxidative decarboxylation of L-malate to produce CO2,
pyruvate and NADPH, was isolated from the leaves of a 2-month-old Aloe vera
L., The level of expression of NADP-ME mRNA and accumulation of NADP-ME (AvME)
protein under salt stress conditions were compared between a tolerant aloe, Aloe
vera L. and a sensitive aloe, Aloe saponarea Haw. The results
suggested that both the expression of the gene and the accumulation of the
protein were induced in the two kinds of aloe, and the strength was related to
the degree of salt tolerance. Northern blot analysis revealed that the gene for
NADP-malic enzyme in Aloe vera L. (AvME) was induced by high salt,
dehydration, and exogenous abscisic acid (ABA), but not by cold treatment. To
further confirm whether the synthesis of AvME protein was induced with hours of
treatment, Western blot analysis of the samples was conducted. The results
indicated that the induction of AvME protein expression was obvious after 48 h
at high salt and the level was increased with the hours of treatment.
Key
words aloe;
NADP-ME; gene expression; salt tolerance
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