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Exogenous Expression of SOCS Box-deficient Mutant ASB-8 Suppresses the Growth of Lung Adenocarcinoma SPC-A1 Cells

  • Post author By abbs

Exogenous
Expression of SOCS Box-deficient Mutant ASB-8 Suppresses the Growth of Lung
Adenocarcinoma SPC-A1 Cells

LIU Yong-Zhong, LI Jin-Jun*, ZHANG Feng-Rui, SHEN Yan1,
YAO Ming, WAN Da-Fang, GU Jian-Ren*

( State
Key Laboratory of Oncogenes and Related Genes, Shanghai Jiaotong University
Cancer Institute, Shanghai 200032, China;
1 Shanghai Research Center of Biotechnology, the Chinese Academy
of Sciences, Shanghai 200233, China )

Abstract        ASB-8
is a new member of the human ankyrin repeat and SOCS box containing protein
family (ASB). This report deals with the expression of ASB-8 protein in lung
carcinoma tissue, as well as its biological effect on the proliferation and
growth of lung cancer cell line SPC-A1. ASB-8 was expressed in pT7-450
expression vector, and an anti-ASB-8 rabbit polyclonal antibody was prepared.
The expression of ASB-8 protein in lung carcinoma tissue was detected using
immunohistochemistry. The growth characteristics of the lung adenocarcinoma
SPC-A1 cells expressing either exogenous ASB-8 or ASB-8DSB (SOCS box-deficient) was
studied by both in vitro cell growth curve and in vivo nude mouse tumor
formation assay. Immunohistochemical staining detected positive reaction of
96.8% (30/31) showing that ASB-8 was highly expressed in lung cancer tissues;
however, the expression of ASB-8 was lower, or even no expression in
noncancerous lung tissues. Significant growth inhibition was observed in SPC-A1
cell line expressing ASB-8DSB on day 4 and persisted to day 6, compared with mock transfected
cells (P<0.01). No difference in the growth properties was observed between ASB-8 and mock transfected cells. In vivo study also showed that
tumor formation of ASB-8DSB expressing cells was significantly inhibited as compared with
that of the control group (P<0.01). Therefore, ASB-8 may play an important role in growth and proliferation of lung cancer, possibly as positive regulator.

Key words     ASB-8
gene; ankyrin repeat; SOCS box; lung cancer

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e-mail, [email protected]