The Effects of Inhibiting P18INK4C Expression on the
Invasion of Gastric Adenocarcinoma Cell Line

WANG Chu, WANG
Jian-Hua, LI Feng, LU Jian*

(
Department of Biochemistry and Molecular Biology, Research Center for Human
Gene Therapy, Shanghai Second Medical University, Shanghai 200025, China )

Abstract             Using
cDNA microarray with double dots of 4096 human genes, P18INK4C, a member of
CKI, was found down-regulated in a gastric adenocarcinoma metastatic cell line
(RF-48), compared with the corresponding primary cancer cell line (RF-1), which
implied that P18INK4C might be involved in cell invasion and metastatic
progression of human gastric adenocarcinoma. Antisense RNA expression plasmid
was applied to inhibit P18INK4C expression to study the effect of decreased
P18INK4C expression on cell migration, invasion and proliferation ability and
cell cycle of RF-1. Results showed that inhibition of P18INK4C expression could
obviously enhance cell invasion ability of RF-1, but had little effect on its
cell cycle and cell migration and proliferation ability. These results implied
that P18INK4C might play a pivotal role in regulating cell invasion, rather
than regulating cell cycle and proliferation in the progression of human
gastric adenocarcinoma as expected before.

Key words        P18INK4C;
human gastric adenocarcinoma; invasion and metastasis

*Corresponding
author: Tel, 86-21-63846590-776441; e-mail,
[email protected]