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ISSN
1672-9145
Acta Biochim Biophys Sin
2004, 36(10): 656–660
CN 31-1940/Q
The cDNA and Genomic DNA Organization of a Novel Toxin SHT-I from
Spider Ornithoctonus huwena
Peng QIAO, Xiao-Pan ZUO, Zhi-Fang CHAI, and Yong-Hua JI*
Institute of
Physiology, Shanghai Institutes for Biological Sciences, Chinese Academy of
Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai 200031,
China
Abstract In present study the
full-length cDNA of a novel toxin from the venom gland of spider Ornithoctonus
huwena, named as SHT-I, has been cloned using the strategy of rapid
amplification of cDNA ends, and then the whole genomic sequence of SHT-I
(Selenocosmia huwena toxin-I) was determined using sequence-specific
primers synthesized based on the acquired 3‘ and 5‘ ends of SHT-I
cDNA sequence. It is unexpectedly found that intron was lacking in the genomic
sequence of SHT-I. The result might evoke an interesting question whether the
gene code of inhibitor cystine-knot peptides from spider venom is distinct from
that of those known toxic peptides of scorpion and cone snail.
Key words Ornithoctonus huwena spider; cDNA;
genomic DNA; peptide toxin; ICK motif
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Received: June 25, 2004 Accepted: September 10,
2004
The work was supported by the grants from the National Basic
Research Program of China (1999054001), and partially the National Nature
Science Foundation of China (30270428)
*Corresponding author: Tel/Fax, 86-21-54920300; E-mail, [email protected]