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ISSN
1672-9145
Acta Biochim Biophys Sin
2004, 36(10): 661–666
CN 31-1940/Q
NO Level and Endothelial NO Synthase Gene Polymorphism (Glu298Asp)
in the Patients with Coronary Artery Disease from the Turkish Population
Lale AFRASYAP* and Guler
OZTURK1
Department of
Biochemistry, Faculty of Health, Mugla University, Mugla, Turkey;
1Department of Physiology, Faculty
of Medicine, Maltepe University, Maltepe-Istanbul Turkey
Abstract Nitric oxide is synthesized from L-arginine by endothelial
nitric oxide synthase encoded by eNOS gene. This study was performed to
investigate the relationship between the serum nitric oxide level and eNOS
gene polymorphism in the Turkish population with angiographically diagnosed
coronary artery disease (63.47±9.10 years old, n=250) and control
subjects without any history and/or risk factors of coronary artery disease
(60.71±9.14 years old, n=150). Griess assay and PCR-RFLP analysis were
used to measure the serum nitric oxide metabolites and genotypes, respectively.
It was found that Glu/Glu, Glu/Asp and Asp/Asp genotype frequencies of the eNOS
were 49.3%, 41.3% and 9.3% respectively in the control group, and 45.6%, 41.2%
and 13.2% in the patient group. Serum nitric oxide levels were (32.56±17.26) mM in controls
and (29.84±11.88) mM in patients. Neither the frequencies of the Glu298Asp genotypes
nor the serum nitric oxide levels showed a significant difference between the
groups. There was also no correlation between serum nitric oxide levels and the
frequencies of the eNOS genotypes. Result showed that the coronary
artery disease of the Turkish population seemed to develop without any
alterations in eNOS Glu298Asp genotype frequency and the serum nitric
oxide level.
Key words coronary artery disease;
genetics; nitric oxide; polymorphism; population
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Received: June 14, 2004 Accepted: August 28,
2004
*Corresponding author: Tel, +90 252 212 04 26; Fax, +90 252 212 47
55; E-mail, [email protected]