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ISSN
1672-9145
Acta Biochim Biophys Sin
2004, 36(10): 701–706 CN 31-1940/Q
The Effect of Bovine IFN-a on the Immune Response in Guinea Pigs
Vaccinated with DNA Vaccine of Foot-and-Mouth Disease Virus
Hui-Chen GUO, Zai-Xin LIU*,
Shi-Qi SUN, Qing-Wen LENG1, Dong LI, Xiang-Tao LIU, and Qing-Ge XIE*
Key Laboratory of Animal Virology of Ministry of Agriculture,
Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences,
Lanzhou 730046, China; 1College of Animal Science and Technology, Shihezi University,
Shihezi 832003, China
Abstract In this study, we constructed recombinant plasmid pcDNA3.1/P12X3C3D including
P1, 2A, 3C, 3D and part of 2B gene of FMDV and pcDNA3.1/IFN containing the gene
encoding bovine IFN-a. We inoculated the DNA vaccine
pcDNA3.1/P12X3C3D with or without pcDNA3.1/IFN to evaluate the efficiency of
this DNA vaccine and the immunogenicity of DNA vaccine enhanced by the
co-delivery with pcDNA3.1/IFN. After two times of vaccination with DNA vaccine,
all of guinea pigs were challenged with 103 ID50 FMDV type O. Anti-FMDV antibody levels were detected by ELISA and T
lymphocyte proliferation response was tested by MTT assay. The result shows
that guinea pigs inoculated by pcDNA3.1/P12X3C3D alone or with pcDNA3.1/IFN
generated specific antibodies and induced an FMDV-specific T lymphocyte
proliferation response. FMDV challenge tests showed that one in four guinea
pigs immunized by pcDNA3.1/P12X3C3D with pcDNA3.1/IFN was protected from the
FMDV serotype O infection. This result indicated that the efficiency of the DNA
vaccine was enhanced by co-delivery with pcDNA3.1/IFN. However, the protection rate
was considerably lower than that immunized with conventional FMD vaccine.
Key words foot-and-mouth disease virus (FMDV); DNA vaccine; IFN-a; gene vaccination
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Received: May 21, 2004 Accepted: August 21,
2004
This work was supported by a grant from the Major State Basic
Research Development Program of China (No. G1999011903)
*Corresponding author: Tel, 86-931-8342585; Fax, 86-931-8340799;
E-mail, [email protected] & [email protected]